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Frequently Asked Questions


The taxonomies for the sequences are not what I expected. Why?

BlobTools takes the information from HITS files and sums up bitscores by taxonomic group at each taxonomic rank (see taxonomy and tax-rule). Reasons for not arriving at the correct taxonomy is that the number of spurious hits is greater than number of good hits:

  • Imagine a sequence similarity search yielded the following result:
taxonomic group
score

A

300

B

110

B

108

B

101

  • Although the best hit is to A, the taxonomy for that sequence would be set to B (score-sum = 319). This issue can be solved by setting the parameter [--min_score FLOAT] (default is '0.0')

Frequently Asked Questions